Enhanced intracellular soluble production of 3-ketosteroid-Δ¹-dehydrogenase from Mycobacterium neoaurum in Escherichia coli and its application in the androst-1,4-diene-3,17-dione production

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• 作者：Minglong Shao ; Yaling Chen ; Xian Zhang ; Zhiming Rao ; Meijuan Xu ; Taowei Yang ; Hui Li ; Zhenghong Xu and Shangtian Yang
• 刊名：Journal of Chemical Technology and Biotechnology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：92
• 期：2
• 页码：350-357
• 全文大小：1060K
• ISSN：1097-4660

文摘

The 3-ketosteroid-Δ¹-dehydrogenase (KSDD) expressed in Escherichia coli was mainly in the form of inclusion bodies and the KSDD enzyme activity was at a low level. Therefore, the regulation of process conditions and supplementation of natural osmolytes were carried out to improve the soluble expression of KSDD in E. coli.