The enhanced production of 11α-hydroxyandrosta-1,4-diene-3,17-dione based on the application of organic silica hollow spheres in the biotransformation of β-sitosterol

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• 作者：Shaofeng Rong ; Jingwen Wang ; Qianqian Li ; Shimin Guan ; Baoguo Cai ; Shuo Zhang and Jing Hu
• 刊名：Journal of Chemical Technology and Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：92
• 期：1
• 页码：69-75
• 全文大小：374K
• ISSN：1097-4660

文摘

11α-hydroxyandrosta-1,4-diene-3,17-dione (11α-OH-ADD) serves as an important steroid intermediate for many hormone pharmaceuticals. However, the poor aqueous solubility of the β-sitosterol which is the substrate hinders its productivity efficiency. This paper reports a new method of biotransformation of β-sitosterol to 11α-OH-ADD by mixed cultivation of Mycobacterium MF006 and Aspergillus ochraceus MF007 with organic silica hollow spheres (OSHS) to improve the dispersibility of the substrate based on its excellent adsorption property. The effect of co-cultivation method and OSHS on the biotransformation were evaluated.