DNA Binding Peptide Directed Synthesis of Continuous DNA Nanowires for Analysis of Large DNA Molecules by Scanning Electron Microscope

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• 作者：Kyung-Il Kim ; Seonghyun Lee ; Xuelin Jin ; Su Ji Kim ; Kyubong Jo and Jung Heon Lee
• 刊名：Small
• 出版年：2017
• 出版时间：January 11, 2017
• 年：2017
• 卷：13
• 期：2
• 全文大小：1306K
• ISSN：1613-6829

文摘

Synthesis of smooth and continuous DNA nanowires, preserving the original structure of native DNA, and allowing its analysis by scanning electron microscope (SEM), is demonstrated. Gold nanoparticles densely assembled on the DNA backbone via thiol-tagged DNA binding peptides work as seeds for metallization of DNA. This method allows whole analysis of DNA molecules with entangled 3D features.