A novel “top-down” strategy for preparing organosilica micelle encapsulating multiple hydrophobic quantum dots as efficient fluorescent label

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• 作者：Hongxin Liu ; Yuan Liang ; Nana Li and Gang Wu
• 刊名：Crystal Research and Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：52
• 期：2
• 全文大小：1552K
• ISSN：1521-4079

文摘

Organosilica micelle incorporating multiple hydrophobic CdSe/ZnS quantum dots (QDs) was prepared by a surfactant free microemulsion approach assisted by ultrasonication. The size of emulsion droplets along with their QDs loading amount was controlled and determined by QDs concentration in alkylsilane. Sodium silicate deposition was important in transforming the amorphous micelles into spherical beads with well defined QDs/SiO₂ structure. These QD micelles were subsequently modified with epoxy group by silane coupling and covalently conjugated to transferrin. After incubated with Hela cells, these fluorescent micelles were detected as bright clusters in cells, which demonstrated the efficient cell internalization of these functionalized particles.