Development of a highly sensitive and specific monoclonal antibody based enzyme-linked immunosorbent assay for the detection of a new β-agonist, phenylethanolamine A, in food samples

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• 作者：Danni Jiang ; Biyun Cao ; Meiyu Wang ; Hong Yang ; Kang Zhao ; Jianguo Li ; Mingxin Li ; Lulu Sun and Anping Deng
• 刊名：Journal of the Science of Food and Agriculture
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：97
• 期：3
• 页码：1001-1009
• 全文大小：598K
• ISSN：1097-0010

文摘

All β-agonists are banned as feed additives for growth promotion in animals due to toxic effects on humans after consuming the β-agonist contaminated meats. Phenylethanolamine A (PA) is a newly emerged β-agonist. Thus there is a need to develop highly sensitive and specific analytical methods for the detection of PA in food samples. In this study, the monoclonal antibody (mAb) against PA was produced by hybridoma technology and used for the development of enzyme-linked immunosorbent assay (ELISA).