Novel anti-adipogenic properties of the individual m>transm>8,m>cism>10 conjugated linoleic acid (CLA) isomer in 3T3-L1 adipocytes

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• 作者：Susana V. Martins ; Virgí ; nia M. R. Pires ; Ana P. Madeira ; Mafalda Nascimento ; Cristina M. Alfaia ; Matilde F. Castro ; Graç ; a Soveral ; José ; A.M. Prates and Paula A. Lopes
• 刊名：European Journal of Lipid Science and Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：119
• 期：2
• 全文大小：548K
• ISSN：1438-9312

文摘

In this study, we hypothesized that m>transm>(m>tm>)8,m>cism>(m>cm>)10 conjugated linoleic acid (CLA) isomer possesses anti-adipogenic properties in resemblance to its structural analog, the m>tm>10,m>cm>12 CLA isomer. After chemical synthesis and purification of the m>tm>8,m>cm>10 CLA isomer in our laboratory, we report, for the first time, its individual action on the lipid metabolism of 3T3-L1 adipocytes. Cells were supplemented with 50 μM of linoleic acid (LA), m>tm>10,m>cm>12, or m>tm>8,m>cm>10 during 11 days of differentiation. Intracellular triacylglycerols (TAG) content was affected by fatty acid treatments. The m>tm>8,m>cm>10 CLA isomer reduced TAG content but in a lower magnitude, three times less, than the m>tm>10,m>cm>12 isomer. The supplementation with LA increased TAG storage thus reversing CLA isomers effect. The fatty acid profile of treated adipocytes showed that the m>tm>8,m>cm>10 CLA isomer incorporation was identical to the m>tm>10,m>cm>12. The mRNA expression of several genes involved in lipogenesis, lipolysis, fatty acid β-oxidation, and adipocytes differentiation underlying anti-adipogenic action was determined. The m>tm>10,m>cm>12 CLA isomer decreased gene expression levels related to lipogenesis but did not up-regulate HSL and PPARA associated with lipolysis and fatty acid β-oxidation, respectively. The m>tm>8,m>cm>10 CLA isomer increased SCD mRNA level without affecting delta 9 desaturase index-16, an indicator of SCD enzyme activity. Taken together, our results indicate that the m>tm>8,m>cm>10 CLA isomer, tested individually on lipid metabolism of 3T3-L1 adipocytes, is anti-adipogenic.