Development and utilization of a new chemically-induced soybean library with a high mutation density

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• 作者：Zhongfeng Li ; Lingxue Jiang ; Yansong Ma ; Zhongyan Wei ; Huilong Hong ; Zhangxiong Liu ; Jinhui Lei ; Ying Liu ; Rongxia Guan ; Yong Guo ; Longguo Jin ; Lijuan Zhang ; Yinghui Li ; Yulong Ren ; Wei He ; Ming Liu ; Nang Myint Phyu Sin Htwe ; Lin Liu ; Bingfu Guo ; Jian Song ; Bing Tan ; Guifeng Liu ; Maiquan Li ; Xianli Zhang ; Bo Liu ; Xuehui Shi ; Sining Han ; Sunan Hua ; Fulai Zhou ; Lili Yu ; Yanfei Li ; Shuang Wang ; Jun Wang ; Ruzhen Chang and Lijuan Qiu
• 刊名：Journal of Integrative Plant Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：59
• 期：1
• 页码：60-74
• 全文大小：2617K
• ISSN：1744-7909

文摘

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M₂ lines, was developed. Over 1,000 M_4 (5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.