Dual Fluorescent- and Isotopic-Labelled Self-Assembling Vancomycin for in vivo Imaging of Bacterial Infections

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• 作者：Dr. Cuihong Yang ; Dr. Chunhua Ren ; Jie Zhou ; Dr. Jinjian Liu ; Yumin Zhang ; Dr. Fan Huang ; Prof. Dr. Dan Ding ; Prof. Dr. Bing Xu and Prof. Dr. Jianfeng Liu
• 刊名：Angewandte Chemie
• 出版年：2017
• 出版时间：February 20, 2017
• 年：2017
• 卷：129
• 期：9
• 页码：2396-2400
• 全文大小：3008K
• ISSN：1521-3757

文摘

The increase of bacterial resistance demands rapid and accurate diagnosis of bacterial infections. Biosurface-induced supramolecular assembly for diagnosis and therapy has received little attention in detecting bacterial infections. Herein we present a dual fluorescent-nuclear probe based on self-assembly of vancomycin (Van) on Gram-positive bacteria for imaging bacterial infection. A Van- and rhodamine-modified peptide derivative (Rho-FF-Van), as the imaging agent, binds to the terminal peptide of the methicillin-resistant staphylococcus aureus (MRSA) and self-assembles to form nanoaggregates on the surface of MRSA. In an in vivo myositis model, Rho-FF-Van results in a significant increased fluorescence signal at the MRSA infected site. Radiolabeled with iodine-125, Rho-FF-Van shows strong radioactive signal in the MRSA-infected lungs in a murine model. This novel dual fluorescent and nuclear probe promises a new way for in vivo imaging of bacterial infections.