Oriented Protein Nanoarrays on Block Copolymer Template

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• 作者：Lei Shen and Jintao Zhu
• 关键词：block copolymer ; immobilization ; orientation ; protein nanoarray
• 刊名：Macromolecular Rapid Communications
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：37
• 期：6
• 页码：494-499
• 全文大小：920K
• ISSN：1521-3927

文摘

Here, a simple yet robust method is developed to fabricate oriented protein nanoarrays by employing a block copolymer (BCP) template, which presents nano-scaled spot areas at high-density arrays. Unlike the conventional BCP nanolithography, the BCP platform described here resists nonspecific protein adsorption and prevents the denaturation of immobilized proteins in aqueous solution. The orderly arranged array areas are functionalized by linking chemistry which allows for the precise control of protein orientation. This approach allows us to generate potentially oriented protein nanoarrays at high-density array spots, which is useful for miniaturized nanoarrays within high-throughput proteomic applications.