Complete Genome Sequence of a Novel Wild tomato mosaic virus Isolate Infecting Nicotiana tabacum in China

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• 作者：Hangjun Sun ; Wei Liu ; Jinguang Yang ; Fenglong Wang ; Lili Shen ; Ying Li ; Dongmei Sun and Yumei Qian
• 刊名：Journal of Phytopathology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：164
• 期：9
• 页码：686-690
• 全文大小：218K
• ISSN：1439-0434

文摘

Virus particles of approximately 740–760 nm in length and 13 nm in diameter were observed from a diseased Nicotiana tabacum (tobacco) plant in Sichuan Province, China. The complete genomic sequence of the virus isolate XC1 was determined to contain 9659 nucleotides without 3′ terminal poly(A) tail. XC1 has a genome typical of members of the genus Potyvirus, encoding a large polyprotein of 3075 amino acids. Putative proteolytic cleavage sites and a number of well characterized functional motifs were identified by sequence comparisons with those of known potyviruses. Sequence comparison revealed that XC1 shared the highest level of nucleotide sequence identity (76.5%) with Wild tomato mosaic virus (WTMV). Phylogenetic analysis showed that XC1 was closely related to the WTMV Guangdong isolate with an identity of 94.3% between CP gene sequence of the two viruses. We thus named XC1 WTMV-XC-1 as a novel isolate of WTMV. The full sequence of WTMV-XC-1 may serve as a basis for future investigations on the gene diversity of WTMV.