Simultaneous quantitation of the diastereoisomers of scholarisine and 19-epischolarisine, vallesamine, and picrinine in rat plasma by supercritical fluid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study

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• 作者：Zhichao Yang ; Lingxia Sun ; Chunsu Liang ; Yongwei Xu ; Jianming Cao ; Yan Yang and Jingkai Gu
• 刊名：Journal of Separation Science
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：39
• 期：13
• 页码：2652-2660
• 全文大小：711K
• ISSN：1615-9314

文摘

Dengtaiye tablet has been used to treat chronic bronchitis cough. Scholarisine, 19-epischolarisine, vallesamine, and picrinine are the representative constituents of Dengtaiye. A rapid and sensitive assay based on supercritical fluid chromatography with tandem mass spectrometry has been developed and validated for the determination of the diastereoisomers of scholarisine and 19-epischolarisine, vallesamine, and picrinine in rat plasma using lamotrigine as internal standard. The analysis in a run time of only 6 min was performed on an ACQUITY UPC² Trefoil^TM BEH 2-EP column (3.0 × 150 mm, 2.5 μm) at 50ºC. The mobile phase consisting of carbon dioxide and methanol (2 mM ammonium formate) was performed as follows: 15% methanol (2 mM ammonium formate) maintained at 0–2 min, 15–19% methanol (2 mM ammonium formate) at 2–4 min, 19–15% methanol (2 mM ammonium formate) at 4–6 min. The flow rate was 1.50 mL/min. The assay was linear over the concentration ranges 50–10000 pg/mL for scholarisine, 19-epischolarisine, vallesamine, and picrinine with corresponding lower limits of quantitation of 50 pg/mL. Intra- and interday precisions were in the range 1.42–12.85% with accuracies in the range –11.71–2.48%. The method was successfully applied to a pharmacokinetic study involving a single oral administration of 108 mg/kg Dengtaiye tablet to rats.