Development of a DNA-Templated Peptide Probe for Photoaffinity Labeling and Enrichment of the Histone Modification Reader Proteins

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• 作者：Xue Bai ; Dr. Congcong Lu ; Dr. Jin Jin ; Shanshan Tian ; Zhenchang Guo ; Pu Chen ; Dr. Guijin Zhai ; Shuzhen Zheng ; Prof. Xiwen He ; Dr. Enguo Fan ; Prof. Yukui Zhang and Prof. Kai Zhang
• 刊名：Angewandte Chemie International Edition
• 出版年：2016
• 出版时间：July 4, 2016
• 年：2016
• 卷：55
• 期：28
• 页码：7993-7997
• 全文大小：1867K
• ISSN：1521-3773

文摘

Histone post-translational modifications (HPTMs) provide signal platforms to recruit proteins or protein complexes to regulate gene expression. Therefore, the identification of these recruited partners (readers) is essential to understand the underlying regulatory mechanisms. However, it is still a major challenge to profile these partners because their interactions with HPTMs are rather weak and highly dynamic. Herein we report the development of a HPTM dual probe based on DNA-templated technology and a photo-crosslinking method for the identification of HPTM readers. By using the trimethylation of histone H3 lysine 4, we demonstrated that this HPTM dual probe can be successfully utilized for labeling and enrichment of HPTM readers, as well as for the discovery of potential HPTM partners. This study describes the development of a new chemical proteomics tool for profiling HPTM readers and can be adapted for broad biomedical applications.