An on-chip electroosmotic (EO) micropump (EOP) was embedded in a microchannel combined with a light-addressable potentiometric sensor (LAPS). The flow direction and the flow rate of EO flows on the LAPS chip could be controlled by the polarity and the magnitude of the applied DC voltage. The EOP could be used as an actuator to generate a sample droplet of about 1 nL and to drive it to the sensing position of a LAPS. A design of a microfluidic system as a (bio-)chemical analysis device for measuring a small volume of sample is proposed.