Direct Electrochemical Sensing of Phenazine-1-carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T Using Disposable Screen-printed Carbon Electrode

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• 作者：Asif Hameed ; Hong-Wei Pi ; Shih-Yao Lin ; Wei-An Lai ; Li-Sen Young ; You-Cheng Liu ; Fo-Ting Shen and Chiu-Chung Young
• 关键词：Phenazine ; Cyclic voltammetry ; Electropolymerization ; Preanodization ; Pseudomonas aeruginosa
• 刊名：Electroanalysis
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：28
• 期：4
• 页码：846-853
• 全文大小：1978K
• ISSN：1521-4109

文摘

A novel electrochemical approach for direct recognition of antibiotic phenazine-1-carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen-printed carbon electrode (SPCE*-PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion-controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*-PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057^T was investigated successfully using present single run approach.