Efficient Synthesis of Topologically Linked Three-Ring DNA Catenanes

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• 作者：Qi Li ; Guangqi Wu ; Dr. Wei Wu and Prof. Dr. Xingguo Liang
• 刊名：ChemBioChem
• 出版年：2016
• 出版时间：June 16, 2016
• 年：2016
• 卷：17
• 期：12
• 页码：1127-1131
• 全文大小：1211K
• ISSN：1439-7633

文摘

Topologically controlled DNA catenanes are promising elements for the construction of molecular machines but present a significant effort in DNA nanotechnology. We report an efficient approach for preparing linear three-ring catenanes (L3C) composed of single-stranded DNA. The linking number was strictly controlled by using short complementary regions (6 nt) between each two DNA rings. High efficiency of forming three-ring catenanes (yield as high as 63 %) was obtained by using an 80 nt oligonucleotide as the scaffold to draw close the three pre-rings for hybridization between short complementary DNA. After assembly, three pre-rings were closed by DNA ligation using three 12 nt oligonucleotides as splints to form interlocked three-ring catenanes. L3C nanostructures were imaged in air by AFM: the catenane exhibited a smooth circular shape and was arranged in a line with well-defined structure, as expected.