Assembly of a Highly Stable Luminescent Zn₅ Cluster and Application to Bio-Imaging

详细信息    查看全文

• 作者：Prof. Dr. Ming-Hua Zeng ; Dr. Zheng Yin ; Ze-Hui Liu ; Prof. Dr. Hai-Bing Xu ; Ying-Chun Feng ; Yue-Qiao Hu ; Li-Xian Chang ; Dr. Yue-Xing Zhang ; Prof. Dr. Jin Huang and Dr. Mohamedally Kurmoo
• 刊名：Angewandte Chemie
• 出版年：2016
• 出版时间：September 12, 2016
• 年：2016
• 卷：128
• 期：38
• 页码：11579-11583
• 全文大小：1712K
• ISSN：1521-3757

文摘

The assembly sequence of the coordination cluster [Zn₅(H₂Lⁿ)₆](NO₃)₄]⋅8 H₂O⋅2 CH₃OH (Zn₅, H₃Lⁿ=(1,2-bis(benzo[d]imidazol-2-yl)-ethenol) involves in situ dehydration of 1,2-bis(benzo[d]imidazol-2-yl)-1,2-ethanediol (H₄L) through the formation of the [Zn(H₃L)₂]⁺ monomer, dimerization to [Zn₂(H₃L)₂]⁺, dehydration of the ligand to [Zn₂(H₂Lⁿ)₂]⁺, and the final formation of the pentanuclear cluster. The cluster has the following special characteristics: 1) high stability in both refluxing 37 % HCl and 27 % NH₃, 2) low cytotoxicity, and 3) pH-sensitive fluorescence in the visible-to-near-infrared (Vis/NIR) region in the solid state and in solution. We have applied it as a fluorescent probe both in vivo and in vitro. Its H-bonding ability is the key to its affinity and selectivity for imaging lysosomes in HeLa cells and tumors in male BALB/C mice. It provides a new type of sensitive and biocompatible fluorescent probe for detecting small tumors (13.5 mm³).