Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles

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• 作者：David Garenne ; Dr. Laure Beven ; Dr. Laurence Navailles ; Prof. Fr&eacute ; d&eacute ; ric Nallet ; Dr. Erick J. Dufourc and Dr. Jean-Paul Douliez
• 刊名：Angewandte Chemie International Edition
• 出版年：2016
• 出版时间：October 17, 2016
• 年：2016
• 卷：55
• 期：43
• 页码：13475-13479
• 全文大小：1886K
• ISSN：1521-3773

文摘

Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.