Maternal transfer and protection role in zebrafish (Danio rerio) offspring following vaccination of the brood stock with a live attenuated Vibrio anguillarum vaccine

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• 作者：Ni Ye ; Haizhen Wu and Yuanxing Zhang
• 刊名：Aquaculture Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：47
• 期：11
• 页码：3667-3678
• 全文大小：456K
• ISSN：1365-2109

文摘

In this study, expressions of some immune parameters among embryos during different development stages from immunized and mock-immunized female fish were compared at day 14 post immunization of the zebrafish brood stock vaccinated with the live attenuated Vibrio anguillarum vaccine MVAV6203. It was found that transcriptional levels of innate immune cell gene markers including spi.1, l-plastin, mpx and gata-1 as well as the myeloid transcription factor c/ebpβ were up-regulated and expressed earlier in embryos of immunized female zebrafish than naive embryos during early development stage. Investigation through ELISA revealed an increase in antibody level in egg cytosol of the female zebrafish following immunization prior to breeding, which was transferred to larvae through eggs. Rise of specific antibody against wild-type V. anguillarumMVM425 was detected at hour 24 post fertilization (p.f.) eggs from immunized brood stock. Extracts of the newly fertilized eggs from immunized zebrafish also showed a higher capability of killing V. anguillarum MVM425. Moreover, larvae from immunized zebrafish also showed a higher capability of resisting the V. anguillarum MVM425 from proliferation after immersion challenge. Interestingly, the higher content of total IgM and specific antibody in eggs and larvae at 24, 48 and 72 h p.f. were positively correlated with their higher antibacterial activity against V. anguillarum MVAV425. Our results showed that, the development of immune cells was enhanced and the maternally derived antibody could protect early embryos and larvae from attack of specific pathogens via vaccination with a live attenuated vaccine.