Dose-dependent protective effects of dietary selenium on abalone Haliotis discus hannai Ino against the toxicity of waterborne copper

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• 作者：Yanju Lei ; Wei Xu ; Yanjiao Zhang ; Huihui Zhou ; Wenbing Zhang and Kangsen Mai
• 刊名：Aquaculture Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：47
• 期：10
• 页码：3215-3223
• 全文大小：192K
• ISSN：1365-2109

文摘

This study was designed to assess the protective effects of dietary selenium (Se) on abalone Haliotis discus hannai Ino against the toxicity of waterborne copper (Cu). A 60-day feeding trial was conducted in a static water system for abalone (initial weight: 3.17 ± 0.01 g) exposed to 0.02 mg L⁻¹ of waterborne Cu. The animals were fed one of the three experimental diets with 0.10, 1.31 and 4.20 mg kg⁻¹ of Se from Na₂SeO₃·5H₂O respectively. Results showed that the abalone fed 1.31 mg kg⁻¹ of dietary Se had the lowest Cu concentration in shell, muscle, mantle, gill, hepatopancreas and serum. Meanwhile, the significant lowest contents of malondiadehyde and protein carbonyl in hepatopancreas were also found in the treatment with 1.31 mg kg⁻¹ of dietary Se (P < 0.05). In addition, this treatment had significant higher glutathione content and thioredoxin reductase activity in hepatopancreas (P < 0.05). However, the activity of Se-dependent glutathione peroxidase (Se-GPx) was significantly decreased in the treatment with 4.20 mg kg⁻¹ of dietary Se (P < 0.05). In this treatment, the protein carbonyl content in hepatopancreas was significantly higher than that in the group with 1.31 mg kg⁻¹ of dietary Se (P < 0.05). In conclusion, in terms of anti-oxidation and Cu accumulation, the protective effects of dietary Se on abalone against waterborne Cu were dose-dependent. The 1.31 mg kg⁻¹ of dietary Se had this effect, but not 4.20 mg kg⁻¹ of dietary Se. Moreover, the latter increased the oxidative stress in abalone exposed to the waterborne Cu.