Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe
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- 作者：Zhenzhen Hua ; b ; Jian Chena ; Wenying Lia ; b ; Yan Wanga ; b ; Yongxin Lia ; Lijia Sanga ; Juanmin Lia ; b ; Qingfeng Zhanga ; b ; Zafar Hussain Ibupotoa ; Cong Yua ; b ; congyu@ciac.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：MicroRNA ; Duplex-specific nuclease (DSN) ; Fluorescence ; Perylene probe ; Induced aggregation
- 刊名：Analytica Chimica Acta
- 出版年：2015
- 出版时间：1 October 2015
- 年：2015
- 卷：895
- 期：Complete
- 页码：89-94
- 全文大小：869 K
文摘
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A novel label free and fluorescence turn on method for the detection of microRNA.
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A duplex-specific nuclease (DSN) was used for signal amplification.
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Controlled aggregation/disaggregation of a perylene probe was used for assay signal transduction.
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The proposed assay method is simple, convenient, cost effective, sensitive and selective.
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