Optimization of dilution refolding conditions for a camelid single domain antibody against human beta-2-microglobulin
详细信息 查看全文
- 作者：Xiaobo Bao1 ; Li Xu1 ; Xu Lu1 ; Lingyun Jia ; lyj81@dlut.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：sdAb ; single domain antibody ; B2M ; beta-2-microglobulin ; DRA ; dialysis-related amyloidosis ; ESRD ; end-stage renal disease ; HRP ; horseradish peroxidase ; RSM ; response surface methodology ; GSH ; reduced glutathione ; GSSG ; oxidized glutathione ; PFLC ; protein folding liquid chromatography ; ELISA ; enzyme linked immunosorbent assay ; ITC ; isothermal titration calorimetry ; 2-ME ; 2-mercaptoethanol ; PEG ; polyethylene glycol ; IPTG ; isopropyl β-d-1-thiogalactopyranoside ; TMB ; 3 ; 3&prime ; 5 ; 5&prime ; -tetramethy
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：59-66
- 全文大小：1422 K
文摘
- •
Established an effective in vitro refolding method for single domain antibody inclusion bodies.
- •
Dilution refolding conditions for sdAb specific against human beta-2-microglobulin were optimized.
- •
The antigen-binding activity of refolded sdAb was assayed by ELISA and isothermal titration calorimetry.
- •
Provide an important strategy for the recombinant production and application of sdAb.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |