Extending the cross-linking/mass spectrometry strategy: Facile incorporation of photo-activatable amino acids into the model protein calmodulin in Escherichia coli cells
详细信息 查看全文
- 作者：Christine Piotrowski ; Christian H. Ihling ; Andrea Sinz ; andrea.sinz@pharmazie.uni-halle.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：CaM ; calmodulin ; CID ; collision-induced dissociation ; DMEM-LM ; Dulbecco&rsquo ; s Modified Eagle&rsquo ; s Limiting Medium ; DTT ; dithiothreitol ; ESI ; electrospray ionization ; FA ; formic acid ; FPLC ; fast protein liquid chromatography ; HPLC ; high-performance liquid chromatography ; IPTG ; isopropyl-β-d-thiogalactopyranoside ; LB ; lysogeny broth ; LC ; liquid chromatography ; MS ; mass spectrometry ; MS/MS ; tandem mass spectrometry ; OD ; optical density ; SDS&ndash ; PAGE ; sodium dodecyl sulfate polyacrylamide gel
- 刊名：Methods
- 出版年：2015
- 出版时间：1 November 2015
- 年：2015
- 卷：89
- 期：Complete
- 页码：121-127
- 全文大小：1036 K
文摘
- •
Use mineral salts medium for incorporating photo-methionine into proteins in E. coli cells.
- •
Cross-links are created upon UV-A irradiation that give 3D-structural information of proteins and protein complexes.
- •
Facile and stochastic incorporation of photo-methionine into calmodulin.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |