Interfacial electron transfer of glucose oxidase on poly(glutamic acid)-modified glassy carbon electrode and glucose sensing

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• 作者：Xuechou Zhou^a ; ^{zhou1300222@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Bingcan Tan^a ; Xinyu Zheng^a ; Dexian Kong^a ; Qinglu Li^a ; ^b ; ^{lql3388@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Poly(glutamic acid)-modified electrode ; Glucose oxidase ; Interfacial electron transfer ; Glucose sensing
• 刊名：Analytical Biochemistry
• 出版年：2015
• 出版时间：15 November 2015
• 年：2015
• 卷：489
• 期：Complete
• 页码：9-16
• 全文大小：1874 K

文摘

The interfacial electron transfer of glucose oxidase (GOx) on a poly(glutamic acid)-modified glassy carbon electrode (PGA/GCE) was investigated. The redox peaks measured for GOx and flavin adenine dinucleotide (FAD) are similar, and the anodic peak of GOx does not increase in the presence of glucose in a mediator-free solution. These indicate that the electroactivity of GOx is not the direct electron transfer (DET) between GOx and PGA/GCE and that the observed electroactivity of GOx is ascribed to free FAD that is released from GOx. However, efficient electron transfer occurred if an appropriate mediator was placed in solution, suggesting that GOx is active. The PGA/GCE-based biosensor showed wide linear response in the range of 0.5–5.5 mM with a low detection limit of 0.12 mM and high sensitivity and selectivity for measuring glucose.