Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum

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• 作者：Miao Bai^a ; ¹ ; Guo-Shun Yang^a ; ¹ ; Wen-Ting Chen^a ; Zhen-Chuan Mao^b ; ^c ; Hou-Xiang Kang^b ; ^c ; Guo-Hua Chen^b ; Yu-Hong Yang^b ; ^c ; Bing-Yan Xie^b ; ^c ; ^{xiebingyan2003@yahoo.com.cn}
• 关键词：AGO ; Argonaute ; CS ; chilling stress ; CDS ; coding DNA sequence ; CK ; control ; DCL ; Dicer-like protein ; dsRNAs ; double-stranded RNAs ; DS ; drought stress ; HS ; heat stress ; hc-siRNAs ; heterochromatic siRNAs ; ITAG ; International Tomato Annotation Group ; miRNAs
• 刊名：Gene
• 出版年：2012
• 出版时间：10 June, 2012
• 年：2012
• 卷：501
• 期：1
• 页码：52-62
• 全文大小：2675 K

文摘

Dicer, Argonaute and RNA-dependent RNA polymerase form the core components to trigger RNA silencing. Although tomato (Solanum lycopersicum) is a dicotyledon model plant, no systematic analysis and expression profiling of these genes in tomato has been undertaken previously. In this study, seven Dicer-like (SlDCLs), 15 Argonaute (SlAGOs) and six RNA-dependent RNA polymerase (SlRDRs) genes were identified in tomato. These genes were categorized into four subgroups based on phylogenetic analyses. Comprehensive analyses of gene structure, genomic localization and similarity among these genes were performed. Their expression patterns were investigated by means of expression models in different tissues and organs using online data and semi-quantitative RT-PCR. Many of the candidate genes were up-regulated in response to Tomato yellow leaf curl virus infection and abiotic stresses. The expression models of tandem gene duplications among SlDCL2s indicated the DCL2 family plays an important role in the evolution of tomato.