PFTK1 regulates cell proliferation, migration and invasion in epithelial ovarian cancer

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• 作者：Weiwei Zhang^a ; ¹ ; Rong Liu^b ; ¹ ; Chunhui Tang^a ; Qinghua Xi^a ; Shumin Lu^c ; Wenjuan Chen^c ; Lianxin Zhu^d ; Jialin Cheng^c ; Yannan Chen^a ; Wei Wang^a ; Jianxin Zhong^a ; ^{ntfyzjx@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yan Deng^a ; ^{ntfydy@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：PFTK1 ; Epithelial ovarian cancer ; Overexpression
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：85
• 期：Complete
• 页码：405-416
• 全文大小：4023 K

文摘

PFTK1, also named Cyclin-Dependent Kinase 14 (CDK14), is a member of the cell division cycle 2 (CDC2)-related protein kinase family. It is a serine/threonine-protein kinase involved in the regulation of cell cycle progression and cell proliferation. In this study, we investigated the role of PFTK1 in epithelial ovarian cancer (EOC) development. The expression of PFTK1 was detected by Western blot and immunohistochemistry staining, both of which demonstrated that PFTK1 was overexpressed in EOC tissues and cells. Statistical analysis showed the expression of PFTK1 was associated with multiple clinicopathological factors, including tumor grade, FIGO stage, lymph node metastatis, Ki-67 expression and predicted a poor prognosis of EOC patients. With in vitro studies we found that PFTK1 expression was decreased in serum-starved ovarian cancer cells, and progressively increased after serum-re-feeding. Knocking PFTK1 down by small interfering RNA (siRNA) significantly inhibited ovarian cancer cell proliferation, migration and invasion. Taken together, our study suggested that PFTK1 played an important role in ovarian cancer development.