Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids

详细信息    查看全文

• 作者：Rodrigo Ledesma-Amaro ; ^{Rodrigo.Ledesma@grignon.inra.fr" class="auth_mail" title="E-mail the corresponding author} ; Jean-Marc Nicaud ; ^{jean-marc.nicaud@grignon.inra.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：GEMs ; Genome scale metabolic models ; MFA ; metabolic flux analysis ; TAG ; triacylglycerol ; DAG ; diacylglycerol ; CLA ; conjutated linoleic acid ; EPA ; eicosapentanoic acid ; DHA ; docosahexanoic acid ; LA ; linoleic acid ; ALA ; alpha-linoleic acid ; RA ; ricinoleic acid ; DCW ; dry cell weight ; DCA ; dicarboxyilic acid
• 刊名：Progress in Lipid Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：61
• 期：Complete
• 页码：40-50
• 全文大小：1197 K

文摘

One of the most promising alternatives to petroleum for the production of fuels and chemicals is bio-oil based chemistry. Microbial oils are gaining importance because they can be engineered to accumulate lipids enriched in desired fatty acids. These specific lipids are closer to the commercialized product, therefore reducing pollutants and costly chemical steps. Yarrowia lipolytica is the most widely studied and engineered oleaginous yeast. Different molecular and bioinformatics tools permit systems metabolic engineering strategies in this yeast, which can produce usual and unusual fatty acids. Usual fatty acids, those usually found in triacylglycerol, accumulate through the action of several pathways, such as fatty acid/triacylglycerol synthesis, transport and degradation. Unusual fatty acids are enzymatic modifications of usual fatty acids to produce compounds that are not naturally synthetized in the host. Recently, the metabolic engineering of microorganisms has produced different unusual fatty acids, such as building block ricinoleic acid and nutraceuticals such as conjugated linoleic acid or polyunsaturated fatty acids. Additionally, microbial sources are preferred hosts for the production of fatty acid-derived compounds such as γ-decalactone, hexanal and dicarboxylic acids. The variety of lipids produced by oleaginous microorganisms is expected to rise in the coming years to cope with the increasing demand.