Characterization of UDP-glucose dehydrogenase from Pasteurella multocida CVCC 408 and its application in hyaluronic acid biosynthesis
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- 作者：Xin Chua ; b ; chux@im.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Jian Hana ; hanjian148.student@sina.com" class="auth_mail" title="E-mail the corresponding author ; Donghui Guoc ; gdonghui2007@163.com" class="auth_mail" title="E-mail the corresponding author ; Zhenzhou Fua ; 425212527@qq.com" class="auth_mail" title="E-mail the corresponding author ; Weifeng Liua ; liuwfv@163.com" class="auth_mail" title="E-mail the corresponding author ; Yong Taoa ; taoyong@im.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pasteurella multocida ; Hyaluronic acid ; UDP-glucose dehydrogenase ; HA synthase ; Whole-cell catalysis
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：64-70
- 全文大小：1198 K
文摘
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PmuHasB, a highly functional UGDH, was expressed and characterized for the first time.
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PmuHasB was introduced in E. coli for enhanced HA production.
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The highly active and stable PmuHasB was found to be crucial for HA production.
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More efficient whole-cell biocatalysis for HA synthesis was constructed in E. coli.
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