An integrated direct loop-mediated isothermal amplification microdevice incorporated with an immunochromatographic strip for bacteria detection in human whole blood and milk without a sample preparation step
详细信息 查看全文
- 作者：Dohwan Leea ; b ; Yong Tae Kimb ; Jee Won Leeb ; Do Hyun Kimb ; Tae Seok Seob ; seots@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Direct loop-mediated isothermal amplification ; No sample pretreatment ; Valveless ; Colorimetric detection ; Integrated microdevice
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：273-279
- 全文大小：1648 K
文摘
- •
Bacteria in real samples were analyzed by Direct LAMP reactions.
- •
The LAMP amplicons were simply detected by immunochromatographic strips.
- •
All processed were performed on the integrated Direct LAMP-ICS microdevice.
- •
Utilization of a slidable plate does not necessitate microvalves for fluidic control.
- •
S. aureus and E. coli O157:H7 in blood or milk were detected at a single-cell level.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |