Multifunctional Reduced Graphene Oxide (RGO)/Fe₃O₄/CdSe Nanocomposite for Electrochemiluminescence Immunosensor

详细信息    查看全文

• 作者：Ying Yang ; Qiao Liu ; Xing-Pei Liu ; Ping-Zhen Liu ; Chang-Jie Mao ; ^{maochangjie@sina.com" class="auth_mail" title="E-mail the corresponding author} ; He-Lin Niu ; Bao-Kang Jin ; Sheng-Yi Zhang
• 关键词：RGO/Fe3O4/PDDA/CdSe nanocomposite ; Magnetic ; Electrochemiluminescence ; immunosensor ; Interleukin-6
• 刊名：Electrochimica Acta
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：190
• 期：Complete
• 页码：948-955
• 全文大小：1382 K

文摘

A novel multifunctional reduced graphene oxide (RGO)/Fe₃O₄/PDDA/CdSe nanocomposite has been successfully prepared and used as probe for an electrochemiluminescence (ECL) immunosensor of human interleukin-6 (IL-6). The prepared RGO/Fe₃O₄/PDDA/CdSe nanocomposite was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible (UV–vis) absorption spectroscopy, and a vibrating sample magnetometer (VSM). This nanocomposite shows magnetism, ECL properties, and good biocompatibility. Furthermore, the RGO/Fe₃O₄/PDDA/CdSe nanomaterial was applied to develop an ultrasensitive ECL immunosensor for detection of IL-6. The ECL intensity decreased linearly with the IL-6 concentrations in the range 0.002 to 20 ng/mL. Furthermore, the developed ECL immunosensor with high sensitivity, stability and excellent reproducibility can be used for detection of real samples, which make it possible to be used in clinical detection.