Comparison of ultra-high performance supercritical fluid chromatography and ultra-high performance liquid chromatography for the separation of spirostanol saponins
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- 作者：Ling-ling Zhua ; Yang Zhaoa ; Yong-wei Xub ; Qing-long Sunb ; Xin-guang Suna ; Li-ping Kanga ; Ren-yi Yanc ; Jie Zhangc ; Chao Liua ; liuchao9588@sina.com" class="auth_mail" title="E-mail the corresponding author ; Bai-ping Maa ; mabaiping@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Spirostanol saponins ; Ultra-high performance supercritical fluid chromatography ; Ultra-high performance liquid chromatography ; Chromatographic behavior ; Natural products
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 February 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：72-78
- 全文大小：1731 K
文摘
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UHPSFC is a good automatic chromatographic technique and has little in common with normal phase HPLC.
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UHPSFC separates spirostanol saponins varying only in sugar chains or in hydroxyls of aglycones well.
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UHPLC with C18 column separates spirostanol saponins varying only in aglycones well, especially with different double bonds.
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UHPSFC and UHPLC are complementary and could be applied together in separating spirostanol saponins.
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