AhR-mediated activity and contributing chemicals were assessed in total and bioavailable fractions of sediment.
AhR-active chemical burdens occurred ubiquitously with a clear spatial pattern linked to sources.
Targeted PAHs explained < 40% of AhR-mediated activity, which was dominated by labile ligands.
Quantitative relationships were verified for both exposure and effect in the sediment-PDMS-biota system.
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