Infrared-active quadruple contrast FePt nanoparticles for multiple scale molecular imaging

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• 作者：Shang-Wei Chou^a ; Chien-Liang Liu^a ; Tzu-Ming Liu^b ; ^c ; ^{tmliu@ntu.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; Yu-Fang Shen^b ; ^c ; ^d ; Lun-Chang Kuo^b ; ^c ; Cheng-Ham Wu^b ; ^c ; Tsung-Yuan Hsieh^b ; ^c ; Pei-Chun Wu^b ; ^c ; Ming-Rung Tsai^b ; ^c ; Che-Chang Yang^e ; Kai-Yao Chang^e ; Meng-Hua Lu^f ; Pai-Chi Li^g ; Shi-Ping Chen^g ; Yu-Hsin Wang^g ; Chen-Wen Lu^h ; Yi-An Chen^a ; Chih-Chia Huangⁱ ; Churng-Ren Chris Wang^j ; Jong-Kai Hsiao^h ; ^{jongkai@tzuchi.com.tw" class="auth_mail" title="E-mail the corresponding author} ; Meng-Lin Li^e ; ^f ; ^{mlli@ee.nthu.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; Pi-Tai Chou^a ; ^{chop@ntu.edu.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：FePt nanoparticle ; Infrared-active ; Multi-modality contrast ; Multiphoton luminescence ; Photoacoustic responses ; Molecular imaging
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：85
• 期：Complete
• 页码：54-64
• 全文大小：3580 K

文摘

A single nanomaterial with multiple imaging contrasts and functions is highly desired for multiscale theragnosis. Herein, we demonstrate single 1–1.9 μm infrared-active FePt alloy nanoparticles (FePt NPs) offering unprecedented four-contrast-in-one molecular imaging – computed tomography (CT), magnetic resonance imaging (MRI), photoacoustic (PA) imaging, and high-order multiphoton luminescence (HOMPL) microscopy. The PA response of FePt NPs outperforms that of infrared-active gold nanorods by 3- to 5.6-fold under identical excitation fluence and particle concentrations. HOMPL (680 nm) of an isolated FePt NP renders spatial full-width-at-half-maximum values of 432 nm and 300 nm beyond the optical diffraction limit for 1230-nm and 920-nm excitation, respectively. The in vivo targeting function was successfully visualized using HOMPL, PA imaging, CT, and MRI, thereby validating FePt as a single nanomaterial system covering up to four types (Optical/PA/CT/MRI) of molecular imaging contrast, ranging from the microscopic level to whole-body scale investigation.