Aqueous extract of Chrysobalanus icaco leaves, in lower doses, prevent fat gain in obese high-fat fed mice

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• 作者：P.A.S. White^a ; ^{pollyannawhite@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; L.M. Cercato^a ; V.S. Batista^a ; E.A. Camargo^a ; W. De Lucca Jr^b ; A.S. Oliveira^a ; F.T. Silva^a ; T.C. Goes^a ; E.R.A. Oliveira^c ; V.R.S. Moraes^c ; P.C.L. Nogueira^c ; A.M. De Oliveira e Silva^c ; L.J. Quintans-Junior^a ; B.S. Lima^d ; A.A.S. Araú ; jo^d ; M.R.V. Santos^a
• 关键词：Rutin (PubChem CID ; 5280805) ; Quercitrin (PubChem CID ; 5280459) ; Quercetin (PubChem CID ; 5280343) ; Myricitrin (PubChem CID ; 5281673) ; Myricetin (PubChem CID ; 5281672) ; Myricetin 3-O-glucuronide (PubChem CID ; 5487413) ; Gallic acid (PubChem CID ; 46780424) ; Trichloroacetic acid (PubChem CID ; 6421) ; Tiobarbituric acid (PubChem CID ; 2723628) ; Ferrous sulfate (PubChem CID ; 24393)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：17 February 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：92-100
• 全文大小：973 K

文摘

Due to the rise in obesity, the necessity for resources and treatments that could reduce the morbidity and mortality associated to this pandemia has emerged. The development of new anti-obesity drugs through herbal sources has been increasing in the past decades which are being used not only as medicine but also as food supplements. Previous studies with the aqueous extract of Chrysobalanus icaco L (AECI) have demonstrated activity on lowering blood glucose levels and body weight.

Aim of the study

Investigate C. icaco effects in overall adiposity and glycemic homeostasis.

Material and methods

C57BL/6J mice were randomly assigned to standard chow (SC) or high-fat diet (HFD) and treated with AECI in 0.35 mg/mL or 0.7 mg/mL concentrations ad libitum. Food intake, feed efficiency, metabolic efficiency, body, fat pads and gastrocnemius weight, adiposity index, serum lipids, fecal lipid excretion, locomotor activity in the open field test and insulin and glucose tolerance tests were analyzed and compared. The major components of the extract were demonstrated through HPLC and its antioxidant activity analyzed through DPPH and lipid peroxidation.

Results

The AECI in the 0.35 mg/mL concentration did not affect food intake or body weight. However, it promoted lower adipose tissue gain, TG levels, and fecal lipid excretion, increased locomotor activity and lean mass weight, and normalized insulin sensitivity and glucose tolerance. Moreover, AECI showed the presence of myricetin 3-O-glucuronide, rutin, quercitrin and myricitrin and demonstrated high-antioxidant activity.

Conclusions

AECI in lower concentrations can prevent fat storage or enhance fat utilization through the increase of locomotor activity. Also, this reinforces its ability to maintain glucose homeostasis through the normalization of insulin sensitivity and glucose tolerance despite the high-fat diet intake. These activities could be associated to the extract's polyphenol content.