Between Oct 12, 1995, and Feb 5, 2002, 308 patients were identified from two prospective, randomised trials at the Royal Marsden Hospital, London and Sutton, UK, for the radiotherapy cohort and diagnostic biopsies were available for 201 of these patients. Between June 6, 1995, and Nov 4, 2005, 329 patients were identified from the Aarhus University Hospital, Skejby, Denmark, for the prostatectomy cohort; of these, 40 patients were excluded because the tumour was too small to sample (19 patients), because the paraffin block was too thin (19 patients), or because the blocks were missing (two patients), leaving 289 patients for analysis. For patients treated with radiotherapy, increased staining for VEGF (p=0·008) and HIF-1 alpha (p=0·02) expression, but not increased osteopontin expression (p=0·978), were significant predictors of a shorter time to biochemical failure on multivariate analysis, independent of clinical tumour stage, Gleason score, serum PSA concentration, and dose of radiotherapy. For patients treated with surgery, increased staining for VEGF (p<0·0001) and HIF-1 alpha (p<0·0001) expression, and increased osteopontin expression (p=0·0005) were each significantly associated with a shorter time to biochemical failure on multivariate analysis, independent of pathological tumour stage, Gleason score, serum PSA concentration, and margin status.
To our knowledge, this is the largest study of intrinsic markers of hypoxia and angiogenesis in relation to the outcome of radical treatment of localised prostate cancer. Increased expression of VEGF, HIF-1 alpha, and, for patients treated with surgery, osteopontin, identifies patients at high risk of biochemical failure who would be suitable for enrolment into trials of treatment intensification.
Prostate Cancer Research Foundation, London, UK; Danish Cancer Society, Copenhagen, Denmark; Cancer Research UK, London, UK; and National Cancer Research Institute South of England Prostate Cancer Collaborative, London, UK.
Over Journal of Nanjing Medical University |
jrn_sub.gif"" alt=""You are entitled to access the full text of this document"" title=""You are entitled to access the full text of this document"" width=12 height=14""> 48a9a35e8b316473b3ddaf761"">Over Journal of Nanjing Medical University, Volume 21, Issue 4, July 2007, Pages 253-256 Yili Han, Dalin He, Yong Luo, Hepeng Cheng, Guangfeng Zhu Abstract ObjectiveTo evaluate the effect of HIF-1 ± over-expression on angiogenesis in human prostate cancer cells.MethodsLNCaP cells(a human prostate cancer cell line) were transfected with the recombinant plasmid pcDNA3.1(-)-HIF-1 α with Lipofectamine 2000 system. The positive clones were selected by G418 being further confirmed by Western blot and immunofluorescence. The expression levels of VEGF, iNOS and Ang- II were determined. ResultsThe expression of HIF-1 α in the LNCaP/HIF1 α cells was significantly increased in transfected cells, which induced the up-regulation of VEGF, iNOS, whereas Ang- II expression remained un-changed. ConclusionOverexpression of HIF-1 α can induce angiogenesis proteins and may improve the angiogenesis potency of prostate cancer. PDF (240 K) |
Angiogenesis and the tumour hypoxia response in prostat... International Journal of Surgery |
jrn_sub.gif"" alt=""You are entitled to access the full text of this document"" title=""You are entitled to access the full text of this document"" width=12 height=14""> Angiogenesis and the tumour hypoxia response in prostate cancer: A review International Journal of Surgery, Volume 3, Issue 1, 2005, Pages 61-67 P. Sooriakumaran, R. Kaba Abstract The formation of new blood vessels, angiogenesis, is a highly-regulated active process that is critical for the development of the normal and malignant prostate. The vascular endothelial growth factor (VEGF) system assumes a critical role in the angiogenic process. Angiogenesis is a prerequisite for the expansion of solid tumours beyond 1–3 mm3 and is stimulated in response to a hypoxic environment. This review discusses the process of angiogenesis and the key angiogenic mediator, VEGF, and their role in tumour progression and metastasis. A better understanding of the mechanisms behind angiogenesis will ultimately lead to the development of new anti-angiogenic agents in the management of prostate cancer. PDF (151 K) |
View More Related Articles |
480,screenX=10,screenY=10'); outwardWin.focus()"">View Record in Scopus |
Hypoxia in prostate cancer: observation to intervention
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址:北京市海淀区学院路29号 邮编:100083 电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700 |