Fast and sensitive quantification of human liver cytosolic lithocholic acid sulfation using ultra-high performance liquid chromatography-tandem mass spectrometry
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- 作者：Sumit Bansala ; Aik Jiang Laua ; b ; aikjiang.lau@nus.edu.sg" class="auth_mail" title="E-mail the corresponding author
- 关键词：DMSO ; Dimethyl sulfoxide ; DTT ; DL-dithiothreitol ; HPLC-UV ; High-performance liquid chromatography-ultraviolet spectroscopy ; LC-MS/MS ; Liquid chromatography-tandem mass spectrometry ; LCA ; Lithocholic acid ; LCA-S ; Lithocholic acid sulphate ; MgCl2 ; Magnesium chloride ; MRM ; Multiple reaction monitoring ; PAP ; 3&prime ; -Phosphoadenosine 5&prime ; -phosphate ; PAPS ; 3&prime ; -Phosphoadenosine 5&prime ; -phosphosulfate ; SULT ; sulfotransferase ; SULT2A1 ; Sulfotransferase 2A1 ; UPLC&ndash ; MS/MS ; Ultra-high perform
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：1011
- 期：Complete
- 页码：171-178
- 全文大小：1070 K
文摘
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UPLC–MS/MS analysis of human liver cytosolic LCA sulfation.
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The matrix (human liver cytosol) did not interfere with the assay.
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The assay was optimized for Mg, DTT, 2-mercaptoethanol, and PAPS concentrations.
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The assay was linear with respect to amount of enzyme and incubation time.
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Faster and more sensitive than existing LC–MS or radiometric methods.
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