Hepatoprotective effects of Solanum nigrum against ethanol-induced injury in primary hepatocytes and mice with analysis of glutathione S-transferase A1

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• 作者：Fang-Ping Liu^a ; ^{fangpingliu@126.com" class="auth_mail" title="E-mail the corresponding author} ; Xin Ma^a ; Min-Min Li^a ; Zhi Li^a ; Qing Han^a ; Rui Li^a ; Chang-Wen Li^b ; Yi-Cong Chang^a ; Chang-Wei Zhao^a ; Yue-Xia Lin^a
• 关键词：ethanol ; glutathione S-transferase alpha 1 ; hepatic injury ; primary hepatocytes ; Solanum nigrum
• 刊名：Journal of the Chinese Medical Association
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：79
• 期：2
• 页码：65-71
• 全文大小：454 K

文摘

Solanum nigrum is a herbaceous perennial plant, which is widely used in traditional medicine systems for its antioxidant, antiulcerogenic, antitumorigenic, and anti-inflammatory characteristics. The purpose of this study was to investigate the protective effects of S. nigrum against alcoholic liver damage in primary hepatocytes and mice, using glutathione S-transferase alpha 1 (GSTA1) as an indicator.

Methods

Primary hepatocytes were obtained by the inverse perfusion method improved on Seglen two-step perfusion in situ.

Results

In the presence of S. nigrum aqueous extracts (100 μg/mL), no hepatocytic damage was observed in cells treated with ethanol, compared with the model group, and GSTA1 (p < 0.01) was more sensitive than alanine aminotransferase and aspartate aminotransferase (p < 0.05). Mice that received S. nigrum aqueous extracts (150 mg/kg) with ethanol showed marked attenuation of ethanol-induced hepatotoxicity, as evidenced by significant reductions of serum transaminases (p < 0.01), and variation of hepatic oxidative indices (p < 0.05) and GSTA1 (p < 0.05), compared with the model group and mice that received S. nigrum aqueous extracts (200 mg/kg). All the detection indexes were significantly different (p < 0.01) from those of the model group, and the protective effects were almost the same as that of the positive drug group.

Conclusion

These results suggested that S. nigrum has hepatoprotective effects against ethanol-induced injury both in vitro and in vivo, and can protect the integrity of hepatocytes and thus reduce the release of liver GSTA1, which contributes to improved liver detoxification.