Programmed cell death, antioxidant response and oxidative stress in wheat flag leaves induced by chemical hybridization agent SQ-1

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• 作者：Shu-ping WANG^a ; ^{wangshuping2003@126.com" class="auth_mail" title="E-mail the corresponding author} ; Gai-sheng ZHANG^a ; ^{zhanggsh58@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Qi-lu SONG^a ; Ying-xin ZHANG^b ; Ying LI^a ; Jia-lin GUO^a ; Zheng CHEN^a ; Na NIU^a ; Shou-cai MA^a ; Jun-wei WANG^a
• 关键词：wheat flag leaf ; chemical hybridization agent SQ-1 ; programmed cell death ; antioxidant response ; oxidative stress
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：15
• 期：1
• 页码：76-86
• 全文大小：3277 K

文摘

Male sterility induced by a chemical hybridization agent (CHA) is an important tool for utilizing crop heterosis. Leaves, especially the flag leaves, as CHA initial recipients play a decisive role in inducing male sterility. To investigate effects of different treatment times of CHA-SQ-1 used, morphological, biochemical and physiological responses of wheat flag leaves were detected in this study. CHA induced programmed cell death (PCD) as shown in terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) and DNA laddering analysis. In the early phase, CHA-SQ-1 trig-gered organelle changes and PCD in wheat leaves accompanied by excess production of reactive oxygen species (O₂ and H₂O₂) and down-regulation of the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POD). Meanwhile, leaf cell DNAs showed ladder-like patterns on agarose gel, indicating that CHA-SQ-1 led to the activation of the responsible endonuclease. The oxidative stress assays showed that lipid peroxidation was strongly activated and photosynthesis was obviously inhibited in SQ-1-induced leaves. However, CHA contents in wheat leaves gradually reduced along with the time CHA-SQ-1 applied. Young flags returned to an oxidative/antioxidative balance and ultimately developed into mature green leaves. These results provide explanation of the relations between PCD and anther abortion and practical application of CHA for hybrid breeding.