Transcription factor NFY globally represses the expression of the C. elegans Hox gene Abdominal-B homolog egl-5

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• 作者：Hansong Deng ; Yinyan Sun ; Yuxia Zhang ; Xin Luo ; Wenru Hou ; Libo Yan ; Yaoyao Chen ; E Tian ; Jinghua Han ; Hong Zhang
• 关键词：NFY ; egl-5 ; CCAAT box ; Transcription ; C. elegans
• 刊名：Developmental Biology
• 出版年：2007
• 出版时间：15 August 2007
• 年：2007
• 卷：308
• 期：2
• 页码：583-592
• 全文大小：1081 K

文摘

The C. elegans Hox gene egl-5 (ortholog of Drosophila Abdominal-B) is expressed in multiple tissues in the tail region and is involved in tail patterning. In this study, we identify and clone the corresponding C. elegans orthologs of the components of the heterotrimeric transcription factor NFY, nfya-1, nfyb-1 and nfyc-1 and demonstrate that mutations in these components result in the ectopic expression of egl-5 outside of its normal expression domains. The NFYA-1 protein forms a complex with NFYB-1 and NFYC-1, specifically recognizing the CCAAT box. Mutating a CCAAT box in the proximal promoter of egl-5 also leads to the derepression of egl-5, suggesting a direct role for the NFY complex in the regulation of egl-5. In addition, we show that the NFY complex interacts with the MES-2/MES-6 PcG complex in Hox gene regulation. Thus, our studies unravel a physiological function of NFY in establishing the spatially restricted expression pattern of egl-5.