25. Model study of the desiccation induced structural transformations of Group-3 Late Embryogenesis Abundant (G3LEA) proteins expressed in anhydrobiotic organisms

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• 作者：Tempei Shimizu ; Minoru Sakurai ; Takao Furuki ; Hisakazu Mihara ; Tsuyoshi Takahashi ; Yasushi Kanamori ; Takahiro Kikawada ; Takashi Okuda
• 关键词：Cryoprotective dehydration ; Adaptation ; Phospholipid fatty acids ; Trehalose ; Glucose
• 刊名：Cryobiology
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：55
• 期：3
• 页码：331-332
• 全文大小：61 K

文摘

The springtail Megaphorura arctica (Onychiuridae: Collembola) inhabits the arctic and sub-arctic parts of the northern hemisphere where it on a seasonal basis will be exposed to severe cold and desiccating conditions. In the present study we compared how traits of stress resistance differed between two populations of M. arctica that were collected at a high arctic site (Spitsbergen) and a sub-arctic site (Akureyri, Iceland) with contrasting thermal environments. In addition we investigated how cold and desiccation affected the phospholipid fatty acid composition of M. arctica from Spitsbergen. The springtails from Spitsbergen were the most cold tolerant and this was linked to an almost three times higher level of trehalose accumulation during cryoprotective dehydration (15 % and 5 % of tissue dry weight in the Spitsbergen and Iceland populations, respectively). Although cryoprotective dehydration is intimately related to desiccation stress it was shown that M. arctica had a higher mortality when dehydrated over ice (−10 or −20 °C) than when dehydrated at temperatures above 1 °C. Thus, survival was lower after exposure to −10 °C than after exposure to a relative humidity of 91.2 % RH at +1 °C although both treatments led to the same level of dehydration. Exposure to both cold (−10 and −20 °C) and desiccation at +1 °C caused significant changes in the phospholipid fatty acid composition with some similarities. These changes included a decrease in average chain length of the fatty acids due primarily to an increase in the phospholipid fatty acids 16:0 and a decrease in 18:3 and 20:4ω6.