Identification and expression characterization of three Wnt signaling genes in pearl oyster (Pinctada fucata)

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• 作者：Jing Gao ; Jun Liu ; Yi Yang ; Jian Liang ; Jun Xie ; Shiguo Li ; Guilang Zheng ; Liping Xie ; ^{lpxie@mail.tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Rongqing Zhang ; ^{rqzhang@mail.tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dvl ; Dishevelled ; TCF ; T-cell factor ; CKI ; casein kinase I ; GSK3β ; glycogen synthase kinase 3β ; APC ; adenomatous polyposis coli ; LEF ; lymphoid enhancer factor ; ARM ; armadillo ; PCP ; planar cell polarity ; RACE ; rapid amplification of cDNA ends ; ESTs ; expressed sequence tags ; NCBI ; National Center of Biotechnology Information ; D ; day ; PCR ; polymerase chain reaction ; UTR ; untranslated region ; ORF ; open reading frame ; DIX ; Dvl and Axin ; PDZ ; postsynaptic density 95 ; discs large and zonula occludens-1
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：June-July 2016
• 年：2016
• 卷：196-197
• 期：Complete
• 页码：92-101
• 全文大小：2076 K

文摘

The Wnt signaling pathway plays an important role in animal development and in the biomineralization process. At present, although the biomineralization mechanism in pearl oyster (Pinctada fucata) has been extensively studied, there is little research on the Wnt signaling pathway in pearl oyster. To understand the potential role of the Wnt signaling pathway in pearl oyster, we cloned and sequenced three genes from the Wnt signaling pathway in pearl oyster that encode the following proteins: β-catenin, Dishevelled (Dvl) and T-cell factor (TCF). Genomic structure analysis revealed that Pf-β-catenin genomic DNA contained 15 exons, Pf-Dvl genomic DNA contained 16 exons, and Pf-TCF genomic DNA contained 7 exons. Their deduced amino acid sequences all showed the highest identity with homologs in Crassostrea gigas. Yeast two-hybrid analysis verified that Pf-β-catenin interacted with Pf-TCF. These three genes were ubiquitously expressed in seven pearl oyster tissues analyzed with the highest expression in the gill and a certain amount of expression in the mantle, a tissue related to shell formation. After shell notching, the dynamic changes in expression of these three genes showed that they reached a maximum at 4 days, indicating their response to shell regeneration. All three genes were constitutively expressed during five developmental stages of the pearl oyster, with high levels at the early embryonic development stage. Taken together, these results suggested that Pf-β-catenin, Pf-Dvl and Pf-TCF might participate in shell formation and early embryonic and larval development in the pearl oyster.