Human IgE is efficiently produced in glycosylated and biologically active form in lepidopteran cells
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- 作者：Frank Bantleona ; 1 ; Sara Wolfa ; b ; 1 ; Henning Seismannc ; Svend Damd ; Andrea Lorentzene ; Michaela Miehea ; Frederic Jabsa ; Thilo Jakobf ; Melanie Pluma ; b ; 1 ; Edzard Spillnera ; 1 ; e.spillner@eng.au.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：AAL ; Aleuria aurantia lectin ; AP ; alkaline phosphatase CCD ; GNA ; Galanthus nivalis lectin ; HRP ; horse radish peroxidase ; IMAC ; immobilized metal ion affinity chromatography ; Sf9 ; Spodoptera frugiperda ; High Five (BTI-TN-5B1-4) ; Trichoplusia ni
- 刊名：Molecular Immunology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：72
- 期：Complete
- 页码：49-56
- 全文大小：1026 K
文摘
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Human IgE is efficiently expressed and secreted by lepidopteran insect cells.
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The complex glycosylation of the recombinant IgE is shifted toward pauci- and oligomannosidic structures without impairment of function.
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The complex-type glycans appear to be dispensable for structure and function.
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