PENTOXIFYLLINE DIMINISHED ACETALDEHYDE-INDUCED COLLAGEN PRODUCTION IN HEPATIC STELLATE CELLS BY DECREASING INTERLEUKIN-6 EXPRESSION

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• 作者：HERNÁ ; NDEZ ; E. ; CORREA ; A. ; BUCIO ; L. ; SOUZA ; V. ; KERSHENOBICH ; D. ; GUTI&Eacute ; RREZ-RUIZ ; M. C.
• 关键词：pentoxifylline ; hepatic stellate cells ; acetaldehyde ; collagen ; IL-6
• 刊名：Pharmacological Research
• 出版年：2002
• 出版时间：November, 2002
• 年：2002
• 卷：46
• 期：5
• 页码：435-443
• 全文大小：457 K

文摘

The effect of pentoxifylline (PTX), a methylxanthine derivative, on collagen induction and secretion and on the production of mRNA of two fibrogenic cytokines: interleukin-6 and transforming growth factor-β₁ (IL-6 and TGF-β₁) in a rat hepatic stellate cell line (CFSC-2G) exposed to acetaldehyde was studied. CFSC-2G cells were treated with 175μM acetaldehyde for 24h. The cells were then exposed to a medium containing 200μM PTX. Collagen secretion, increased 2.6 times in acetaldehyde treated cells. Cells exposed to acetaldehyde and treated with PTX diminished collagen secretion to control values and decreased α₁(I) collagen mRNA by 15 % . Reverse transcriptase-polymerase chain reaction (RT-PCR) assays of TGF-β₁ mRNA showed no variation in different experimental conditions. However, PTX induced a decrease of 32 % in IL-6 mRNA in acetaldehyde-treated cells. CFSC-2G cells treated with anti-IL-6 monoclonal antibody, 15min before acetaldehyde was added, did not present an increase in α₁(I) collagen mRNA. These results show that PTX inhibits the expression of α₁(I) collagen via the inhibition of IL-6 in acetaldehyde treated cells. The effect herein reported on IL-6 and α₁(I) collagen mRNA adds to the previously described effect of PTX, which could be useful in the fibrogenic process induced by acetaldehyde.