Fluorescent sensors based on [12]aneN₃-modified BODIPY: Discrimination of different biological thiols in aqueous solution and living cells

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• 作者：Yong-Guang Gao^&dagger ; ; Ying Zhang^&dagger ; ; You-Di Shi ; Hu-Jun Hao ; Bing Gong ; Zhong-Lin Lu ; ^{luzl@bnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：BODIPY ; [12]aneN3 ; Thiols ; Sensor
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2016
• 出版时间：1 April 2016
• 年：2016
• 卷：24
• 期：7
• 页码：1550-1559
• 全文大小：2603 K

文摘

Two fluorescent probes, 1 and 2, derived from borondipyrromethene (BODIPY) modified with macrocyclic polyamine [12]aneN₃, were synthesized and applied in the discrimination of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) with absorption and fluorescent spectroscopy in comparison. It was found that Boc-protected 1 showed highly sensitive and selective recognition of GSH over Cys and Hcy; while probe 2 was able to distinguish the three different thiols due to their different reactivities. With its water-solubility, rapid responsiveness, high sensitivity and low cytotoxicity, probe 2 was successfully applied in the fast detection of three biothiols in living cells.