Fluorescent assay for alkaline phosphatase activity based on graphene oxide integrating with λ exonuclease
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- 作者：Xue-Guo Liub ; 1 ; Xiao-Jing Xinga ; 1 ; xingshujing2005@163.com" class="auth_mail" title="E-mail the corresponding author ; Bo Lia ; Yong-Ming Guoa ; Ye-Zhen Zhanga ; Yan Yanga ; Lian-Feng Zhanga ; lfzhang66@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Graphene oxide ; λ Exonuclease ; Fluorescence ; Alkaline phosphatase
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：81
- 期：Complete
- 页码：460-464
- 全文大小：680 K
文摘
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A novel fluorescence sensor for alkaline phosphatase (ALP) is developed.
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This approach is based on graphene oxide coupled with λ exonuclease.
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A low detection limit of 0.19 U/L toward target ALP is obtained.
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Expanding the exploitation of GO-based sensor for protein without specific binding element.
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