Optimized dendrimer-encapsulated gold nanoparticles and enhanced carbon nanotube nanoprobes for amplified electrochemical immunoassay of E. coli in dairy product based on enzymatically induced deposition of polyaniline
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- 作者：Xinai Zhang ; zhangxinai@mail.ujs.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jianzhong Shen ; Haile Ma ; Yuxiang Jiang ; Chenyong Huang ; En Han ; Boshui Yao ; Yunyao He
- 关键词：Dairy product ; E. coli ; Polyaniline ; Electrochemical immunosensor ; Signal amplification
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：666-673
- 全文大小：2251 K
文摘
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The optimal HAuCl4/PAMAM ratio was investigated to prepare the efficient PAMAM(Au) as immunosensing platform.
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The {dAb-CNT-HRP} nanoprobes were designed by exploiting CNTs for loading high contents of HRP.
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The nanoprobes could catalyze oxidation aniline to produce electroactive PAn for electrochemical measurement.
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The amplified electrochemical immunoassay of E. coli in dairy product was developed.
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The method provided a powerful tool to estimate the quality of dairy product.
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