Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin
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- 作者：Jingjun Wua ; c ; 1 ; wujingjun2009@foxmail.com" class="auth_mail" title="E-mail the corresponding author ; Yang Jia ; 1 ; jiyang0326@163.com" class="auth_mail" title="E-mail the corresponding author ; Nan Sua ; sunan12345@126.com" class="auth_mail" title="E-mail the corresponding author ; Ye Lib ; liyeyashi@163.com" class="auth_mail" title="E-mail the corresponding author ; Xinxin Liub ; liuxinxin0412@163.com" class="auth_mail" title="E-mail the corresponding author ; Xiang Meia ; meixianggoodman@126.com" class="auth_mail" title="E-mail the corresponding author ; Qianqian Zhoua ; yoyo_qian@126.com" class="auth_mail" title="E-mail the corresponding author ; Chong Zhanga ; chongzhang@mail.tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xin-hui Xinga ; xhxing@tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：ChonB ; chondroitin B lyase ; CP ; Chinese Pharmacopoeia ; CS ; chondroitin sulfate ; DS ; dermatan sulfate ; E.coli ; Escherishchia coli ; EP ; European Pharmacopoeia ; GAG ; glycosaminoglycan ; GalNAc4S ; 4-O-sulfo-α-d-N-acetylgalactosamine ; GlcNS6S ; 6-O-sulfo-N-sulfo-α-d-glucosamine ; HPLC ; high performance liquid chromatography ; HS ; heparan sulfate ; IdoA ; α-l-iduronic acid ; IdoA2S ; 2-O-sulfo-α-l-iduronic acid ; IPTG ; isopropyl-β-d-1-thiogalactopyranoside ; IU ; international unit ; LOD ; limit of detection ; LOQ
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：25 June 2016
- 年：2016
- 卷：144
- 期：Complete
- 页码：338-345
- 全文大小：997 K
文摘
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Chondroitin B lyase-based analytical methods selectively quantify dermatan sulfate in heparin.
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Simple enzymatic colorimetric method rapidly detected DS above 0.1 mg mL−1 in heparin.
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LOD of the novel enzymatic SAX-HPLC method reached 1.0 μg mL−1.
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Both methods exhibited good linearity, repeatability, precision and recovery.
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