Comparison of three current viral load assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma

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• 作者：Katsoulidou ; Antigoni ; Papachristou ; Eleni ; Petrodaskalaki ; Maria ; Sypsa ; Vana ; Anastassopoulou ; Cleo G. ; et. al.
• 关键词：HIV-1 RNA ; Viral load ; LCx ; Branched DNA ; COBAS Amplicor Monitor v1.5
• 刊名：Journal of Virological Methods
• 出版年：2004
• 出版时间：October, 2004
• 年：2004
• 卷：121
• 期：1
• 页码：93-99
• 全文大小：120 K

文摘

The LCx HIV RNA quantitative assay (Abbott Laboratories, Delkenheim, Germany) was compared with the Versant HIV-1 RNA 3.0 (bDNA) assay (Bayer, Tarrytown, NY) and the COBAS Amplicor HIV-1 Monitor v1.5 test (Roche Diagnostics, Branchburg, NJ), using plasma samples of various viral load levels from HIV-1-infected patients. Considering the lower limit of the linear range of 50copies/ml of both assays, the detection range of the LCx was 127/151 (84.1 % ) versus the 131/151 (86.8 % ) of the bDNA 3.0 assay, while overall agreement between the two assays was 93.4 % (141/151). LCx and bDNA 3.0 results were found to be strongly correlated (r = 0.96). The fitted regression line was described by the equation log₁₀(LCx copies/ml) = 0.05 + 1.06 × log₁₀(bDNA 3.0copies/ml) with 95 % CI for the estimated slope and intercept at 1.01, 1.12 and −0.16, 0.26, respectively. Similarly, the detection range of the LCx was 115/148 (77.7 % ) versus the 128/148 (86.5 % ) of the Monitor v1.5 test. A 91.2 % concordance (135/148) was observed between these two assays at a cut-off of 50copies/ml. LCx and Monitor v1.5 results were highly correlated (r = 0.96). The fitted regression line was described by the equation log₁₀(LCx copies/ml)=0.06 + 1.03 × log₁₀(Monitor v1.5copies/ml) with 95 % CI for the estimated slope and intercept at 0.97, 1.09 and −0.16, 0.28, respectively.