MnSe-loaded g-C₃N₄ nanocomposite with synergistic peroxidase-like catalysis: Synthesis and application toward colorimetric biosensing of H₂O₂ and glucose

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• 作者：Fengmin QiaoAuthor Vitae ; Qingqing QiAuthor Vitae ; Zhenzhen WangAuthor Vitae ; Ke XuAuthor Vitae ; Shiyun Ai ; ^{ashy@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Graphite phase carbon nitride ; Synergistic effect ; Peroxidase ; Colorimetric detection
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：28 June 2016
• 年：2016
• 卷：229
• 期：Complete
• 页码：379-386
• 全文大小：1996 K

文摘

Manganese selenide (MnSe) nanoparticles as nanocatalysts may present enhanced peroxidase-like catalysis if loaded on conductive graphite carbon nitride (g-C₃N₄) supports. In this work, MnSe nanoparticles were successfully loaded on the surface of polymeric g-C₃N₄ as a cocatalyst through a thermal condensation method by calcinating the mixture of pure MnSe, dicyandiamide and cyanuric acid. The morphology and composition of thus-prepared MnSe loaded doped graphite phase carbon nitride nanosheets (MnSe-g-C₃N₄) were characterized by transmission electron microscopy (TEM), high resolution TEM, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra and X-ray photoelectron spectroscopy (XPS). Structural analyses revealed that the MnSe-g-C₃N₄ nanoparticles are composed of ultrathin nanosheets with 20 nm lateral diameter. Compared with original MnSe and g-C₃N₄, the prepared MnSe-g-C₃N₄ nanosheets could achieve higher aqueous stability and, especially, much stronger peroxidase-like catalysis, presumably resulting from the synergetic effects of conductive carbon nitride and lamellate MnSe nanocatalysts effectively loaded. Significantly, MnSe-g-C₃N₄ nanosheets were proved to be novel peroxidase mimetics which could quickly catalyze oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H₂O₂, producing a blue colored solution. Kinetic analysis indicates that the catalytic behavior satisfy typical Michaelis–Menten kinetics and follows a ping-pong mechanism. Colorimetric detections of H₂O₂ and glucose using the MnSe-g-C₃N₄ nanosheets were conducted with high detection sensitivities, demonstrating the feasibility of practical sensing applications. It is therefore believed that our findings in this study could open up the possibility of utilizing MnSe-g-C₃N₄ nanosheets as enzymatic mimics in diagnostics and biotechnology fields.