Development of a simple pyrene-based ratiometric fluorescent chemosensor for copper ion in living cells

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• 作者：Yin-Shuang Wu^aAuthor Vitae ; Chun-Yan Li^a ; ^{lichunyan79@sina.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Yong-Fei Li^bAuthor Vitae ; Dan Li^aAuthor Vitae ; Zhi Li^aAuthor Vitae
• 关键词：Chemosensor ; Ratiometric ; Pyrene ; Excimer ; Copper ion
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：222
• 期：Complete
• 页码：1226-1232
• 全文大小：1465 K

文摘

A simple pyrene compound (1) containing thiophene is synthesized and used as ratiometric fluorescent chemosensor to recognize Cu²⁺. The ratiometric signal change of the chemosensor is on the basis of monomer–excimer conversion of 1 induced by Cu²⁺. As far as we know, it is the first ratiometric chemosensor based on pyrene compound that is developed for Cu²⁺. The analytical performance characteristics of the proposed Cu²⁺-sensitive chemosensor are investigated. The chemosensor can be applied to the quantification of Cu²⁺ with a linear range covering from 1.0 × 10⁻⁷ to 2.0 × 10⁻⁵ M with the detection limit is 2 × 10⁻⁸ M. The experiment results show that the response behavior of 1 toward Cu²⁺ is pH independent under a wide range of pH from 3.0 to 8.0. Most importantly, the fluorescent changes of the chemosensor are remarkably specific for Cu²⁺ in the presence of other metal ions, which meet the selective requirements for practical application. Moreover, the response of the chemosensor toward Cu²⁺ is fast (response time less than 2 min). In addition, the chemosensor has been used to detect Cu²⁺ in water samples and image Cu²⁺ in living cells with encouraging results.