Alternate carbohydrate and nontraditional inducer leads to increased productivity of a collagen binding domain fusion protein via fed-batch fermentation
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- 作者：McKinzie Fruchtla ; 1 ; Joshua Sakonb ; Robert Beitlea ; rbeitle@uark.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：BCIP ; 5-bromo-4-chloro-indolyl phosphate ; BSA ; bovine serum albumin ; CBD ; collagen binding domain ; DCW ; dry cell weight ; EDTA ; ethylenediaminetetraacetic acid ; F ; feeding rate (l/h) ; GST ; glutathione-S-Transferase ; IPTG ; isopropyl-β-d-thiogalactopyranoside ; LB ; Luria Broth ; m ; maintenance coefficient (g/g ; h) ; Ms ; mass flow rate (g/h) ; μ ; specific growth rate (1/h) ; NBT ; nitro-blue tetrazolium ; PBS ; phosphate buffered saline ; PTH-CBD ; parathyroid hormone-collagen binding domain ; SDS-PAGE ; so
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 May 2016
- 年：2016
- 卷：226
- 期：Complete
- 页码：65-73
- 全文大小：1223 K
文摘
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Alternative induction and feed techniques for E. coli was investigated.
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Combination of lactose induction and glycerol feed surpassed IPTG induction results.
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A modified feeding profile that accounts for a reduced substrate uptake rate should be used for glycerol feed.
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